| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Stanton | A | Dowdy, Mason | Johnson, Michael | Kohl, Gabe | Kelley, Leo | 1:10.00 |
| 4 | Park | A | Sharma, Shawn | Northern, David | Sobie, Ryan | Zenteno, Erick | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Fremont | A | Chandrasekera, Ayesh | Sanchez, Daniel | Reynolds, Gabriel | Anselmi, Marco | 1:02.00 |
| 2 | Antioch Upper | A | Goodmann, Sean | Van Alstine, Logan | Dominguez, Roberto | Wagner, Sean | 55.90 |
| 3 | Deer Path | A | Graves, Alex | Graves, Ben | Stohlgren, Martin | Gadbois, Marcelo | 54.00 |
| 4 | Hawthorn North | A | Koonce, Colin | Gitzinger, Christian | Lee, Woojin | Brennan, Connor | 54.00 |
| 5 | Matthews | A | Dynek, Matthew | Bernabe, Antonio | Brown, Nate | Carter, Garrison | 1:02.00 |
| 6 | West Oak | A | Tovar, Charley | Shannon, Danny | Winter, Jasper | Marcos, Alan | 1:03.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Round Lake | A | Young, Tabione | Ayala, Manuel | Acosta, Christopher | Brown, Jamariel | 52.00 |
| 2 | Barrington-Prairie Campus | A | Kilayko, Chris | Lee, Connor | Li, Jonanthan | Berry, Jared | 51.00 |
| 3 | Grayslake | A | Johnson, Connor | Edwards, Maurice | Mendoza, Justin | Gonzalez, Erick | 50.00 |
| 4 | Hawthorn South | A | Aranda, E | Simes, Cam | Lindley, Carson | V, Omar | 51.00 |
| 5 | Carl Sandburg | A | Walter, Eli | Shaltiel, Jackson | Chen, Matt | Ambriz, Erik | 51.30 |
| 6 | John T Magee | A | Marquez, Teo | Ros, Alex | Valdivia, Isaac | Hermosa, Rodrego | 53.00 |